Prevalence of herpes simplex virus infection in blood donors in some areas of China

HOU Ying;GAO Zhan;Xu Min;ZHANG Yu;HE Miao;Chinese Academy of Medical Sciences,Peking Union Medical College,Institute of Blood Transfusion;  

Objective To investigate the prevalence of herpes simplex virus in Chinese blood donors,the viral load and the detection of the serological antibodies. Methods A quantitative real-time PCR system was established to detect 3,794 HSV DNA in plasma from five Chinese blood centers,from Chongqing,Luoyang,Urumqi,Mianyang and Liuzhou. The viral load of positive samples was measured. At the same time,292 randomly selected plasma from the five blood centers were tested with ELISA method to detect Ig G and Ig M infections. Results In this blood donor population,43 HSV DNA-reactive samples were found,accounting for 1. 13%( 43 /3 794). The detection rate in five blood centers were Chongqing 1. 24%( 20 /1611),Luoyang 0( 0 /624),Urumqi 3. 63%( 14 /413),Mianyang 1. 88%( 9 /479),Liuzhou 0( 0 /667),respectively. The viral load range was 9. 15 × 102- 9. 65 × 104 cp / m L. The median viral load was 3. 53 × 103 cp / m L. In 292 specimens by ELISA,Ig G antibody positive rate was 16. 44%( 48 /292) and Ig M antibody positive rate was 4. 45%( 13 /292).2. 40%( 7 /292) of specimens were seropositive for HSV Ig G and Ig M antibodies. Conclusion Currently,there is a certain prevalence of HSV in Chinese blood donors,but the viral load detected in HSV DNA positive specimens was lower. Whether or not the blood recipient needs to do a HSV detection is worth further exploration.

【CateGory Index】： R440

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