Invalid red blood cell transfusion in patients with peripheral blood T Lymphocyte subgroup and changes in regulatory T-cells level
LI Zhijing;Department of Blood Transfusion,Liaoning University of Traditional Chinese Medicine Hospital;
Objective To investigate the peripheral blood T lymphocyte subsets in invalid red blood cell transfusion cases and to observe regulatory T cells( T-reg cells) level changes. Methods Flow cytometry was used before and after respectively to detect the peripheral blood T lymphocyte subsets( CD3~+,CD4~+,CD8~+,CD4~+/ CD8~+,CD4~+CD25~+Treg) and their levels in 30 patients with invalid red blood cell infusion. Results In invalid transfusion cases,the number of CD3~+T cell and CD4~+T cells( P 0. 05) and the CD4~+/ CD8~+ratio rose before blood transfusion. The percentage of CD8~+T cells did not change( P 0. 05). The CD4~+CD25~+T-reg levels fell before a blood transfusion. In the successful transfusion group,the level of T lymphocyte subsets did not change before and after blood transfusion( CD3~+,CD4~+,CD8~+,CD4~+/ CD8~+,CD4~+CD25~+Treg)( P 0. 05). Conclusion In invalid transfusion cases,patients may harbor abnormal CD4~+CD25~+T-reg cells,which can lead to abnormal activation of T cells and may contribute to the failure of red blood cell transfusion.
【CateGory Index】： R457.1