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《Chinese Journal of Blood Transfusion》 2018-03
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The protective impact of trehalose on lyophilized red blood cells

LI Wenhui;WANG Jue;LI Ling;SONG Ning;LIU Zhong;Institute of Blood Transfusion,Chinese Academy of Medical Sciences & Peking Union Medical College;  
Objective To investigate the protective mechanism of trehalose on the cell membrane of lyophilized red blood cells( RBCs) by exploring its influence on the recovery rate and water residuals. Methods RBCs were incubated with various concentration of trehalose solution( 100、200、400、600、800、1 000 mmol/L) and control group were prepared according to conventional methods. Their trehalose content were determined by Trehalose kits. Cells loaded with trehalose were lyophilized with a freeze-dryer. Their residual water content were determined by Karl-Fisher titration and the recovery rates were analyzed with automatic blood cell counter. Results As the concentration of trehalose increased,recovery rates of RBCs kept steady first and then increased; The highest recovery rate is obtained at the concentration of 800 m M. In terms of residual water content,it presents a generally decreasing trend as the the concentration of trehalose increased. The figures for residual water dropped sharply to 3. 47 ± 0. 21% and 2. 43 ± 0. 18% when the concentration reaches to 800 mmol/L and 1 000 mmol/L,respectively. Conclusion Our experiments primarily confirm that the protective mechanism of trehalose on lyophilized red blood cells was water replacement.
【Fund】: 国家自然科学基金(81370669);; 四川省科技新苗子工程项目(2017046)
【CateGory Index】: R457.1
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