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《Chinese Journal of Blood Transfusion》 2018-03
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Effect of IVIG on phagocytosis of human macrophages induced by PMA

HOU Mingxia;YE Shengliang;LIU Fengjuan;LI Dong;MA Li;LI Changqing;Institute of Blood Transfusion,Chinese Academy of Medical Sciences & Peking Union Medical College;  
Objective To investigate the effect of intravenous immunoglobulin( IVIG) on the phagocytosis of human macrophages induced by phorbol 12-myristate 13-acetate( PMA)in vitro and discuss the immunosuppressive effects for autoimmune disease by IVIG. Methods Firstly,human acute monocytic leukemia THP-1 cells were induced to differentiate into macrophages by PMA. Then the effects of different concentrations of IVIG on the phagocytosis of the sensitized erythrocytes by the macrophages were compared by flow cytometry. Results The THP-1 cells were induced by 100 ng/m L PMA for 24 h and successfully differentiated into macrophages. In addition,the phagocytosis of sensitized erythrocytes by the macrophages was completely inhibited when the concentration of IVIG reached10 μg/m L. Conclusion IVIG can effectively inhibit the phagocytosis of macrophages in a dose-dependent manner. This study lay a solid foundation of methodology for improved quality control of IVIG products.
【Fund】: 2016四川省卫生和计划生育委员会科研课题(16ZD044);; 四川省科技厅计划项目(2017JY0064)
【CateGory Index】: R392
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