Application of two-stage enzyme test in the identification of red cell irregular antibodies
ZHANG Lan;LIU Canhua;Nuclear Medicine Department,The People′s Central Hospital of Ji′an;Blood Transfusion Department,The People′s Central Hospital of Ji′an;
Objective To study the application of two-stage enzyme test in the identification of irregular antibody. Methods From January to September 2018, 28 samples screened to have very weak antibodies with uncertian serologic specificity during routine pre-transfusion test were further identified by two-stage enzyme test. The specificity of antibodies was determined by analyzing the the results of antibody identification before and after two-stage enzyme test. Six representative cases were selected. Combined with the results of blood group typing, the adoption of enzyme test on different blood group systems was discussed, along with the application extent of enzyme test.Results Among the 28 samples screened and identified as weakly positive, specificity were identified in 71.43%(20/28) after using enzyme test, among which Rh blood group system antibodies accounted for 60%(12/28), Kidd antibodies for 15%(3/20), Lewis antibodies for 10%(2/20), autoantibodies for 15%(3/20), and antibodies whose specificity remained unknown for 28.57%(8/28). Conclusion Enzyme-IAT test can enhance the ability to detect weak antibodies and clarify the specificity, which helps to ensure the safety of clinical blood transfusion. Autoantibodies, however, precluded the application of enzyme test to some extent, and other methods should be combined for a better diagnosis.
【CateGory Index】： R446.6