Serology characteristics and molecular genetic analysis of CisAB blood group

YANG Qiankun;JIN Huifang;LV Xianping;LIU Aiping;TANG Jinling;Department of Bloood Transfusion, The First Affiliated Hospital of Zhengzhou University;Department of Bloood Transfusion, People′s Hospital of Xinzheng;  

Objective To investigate the characteristics of CisAB and analyze the relationship between their phenotype and genotype and then reach a reasonable transfusion strategy. Methods Blood samples from the family were determined by the method of serology,the preliminary ABO genotype was evaluated with polymerase chain reaction-sequence specific primers(PCR-SSP) technology and ABO gene sequencing.Results Among the six samples included in this study, three samples showed mismatched in forward and reverse blood grouping results, the propositus were serologically typed as having the A_2B_X phenotype, her mother and her bleeding of the umbilicus were typed as having the A_2B and AB phenotype respectively, and their corresponding genotypes contained cisAB01/O01, CisAB01/B101 and cisAB01/O01. Further sequencing analysis of the three samples with the CisAB blood type revealed that a G(261) deletion occurred in exons 6, 467 CT and 803 GC substitutions in exons 7 of CisAB01/O01, while a 297 AG substitution occurred in exon 6 and 467 CT, 796 CA and 803 GC substitutions occurred in exon 7 of CisAB01/B101. Conclusion Genotyping is a more precise method to identify blood subgroups than a routine serological method when dealing with the forward and reverse mismatched blood grouping.

【CateGory Index】： R457.11

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