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《Journal of Beijing Agricultural College》 2006-01
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Optimization of SSR-PCR Amplified Condition in Adzuki Bean

DING Yan-hong,JIN Wen-lin , ZHAO Bo,PU Shao-jing ( Beijing Agricultural College, Beijing 102206,China; Xinjiang Agricultural University Agricultural College, Urumqi 830052, China)  
In this study,we optimizatied SSR-PCR amplified condition on Adzuki bean and analyzed effects of the concentration of Mg2+ ,dNTP,Taq polymerase, primer and DNA on PCR results. It showed that: in 10 μL PCR reaction,2 mmol/L Mg2+ ; 0. 25 mmol/L dNTP; 1 U Taq polymerase,0. 15 mmol/L primer and 20 ng DNA were the best consistence.
【Fund】: 北京市自然科学基金(项目编号:6042006) 农业部948项目(项目编号:2004-Z20)
【CateGory Index】: S521
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