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《Journal of Beijing University of Agriculture》 2018-01
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Isolation,identification and mycelial culture conditions of two wild morels

HAN Peng;CHEN Qingjun;HE Guoqiang;WEI Jinkang;ZHANG Guoqing;WANG Shounan;LIU Zilu;SUN Yue;Key Laboratory of Urban Agriculture(North)Ministry of Agriculture/College of Biological Science and Engineering,Beijing University of Agriculture;Plant Science and Technology College,Beijing University of Agriculture;Beijing Agricultural Technology Extension Station;  
【Objective】The present study was to obtain wild morels resources and their optimal mycelia culture conditions.【Methods】Pure culture species were obtained using tissue separation and purification method.They were subsequently identified based on morphological and internal transcribed spacer(ITS)identification.The optimal culture conditions of mycelia were determined based on mycelial growth rate and vigour.【Results】Two pure culture morels from Beijing and Shanxi provinces were numbered as YBJ and YSX,and subsequently identified as M.esculentaand M.deliciosa,respectively.Optimal mycelia culture conditions of YBJ were:optimal carbon source of soluble starch and sorbitol,optimal nitrogen source of peptone,optimal C/N ratio range of 10/1-30/1,optimal temperature of 24℃,and optimal pH of 8.0.Optimal mycelia culture conditions of YSX were:optimal carbon source of maltose,optimal nitrogen source of urea,optimal C/N ratio of 60/1,optimal temperature of 24 ℃,and optimal pH of 8.0.【Conclusion】Two pure culture strains were obtained from wild morels.Taxonomic status and their optimal culture conditions were further determined.The present study could provide a theoretical basis for further applications of morels resources.
【Fund】: 北京市农业科技项目(PXM2017_036204_000011);; 北京市科技新星项目(xx2015B025)
【CateGory Index】: S646.7
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