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《Journal of Beijing University of Agriculture》 2018-01
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The remission effect and mechanism of lysine on rat jejunal mucosal injury and autophagy

LIU Yilin;HUANG Liqing;LIU Beihua;JIN Na;ZOU Wenshu;CUI Weidong;GAO Qian;CHEN Changzeng;LIU Fenghua;Animal Science and Technology College,Beijing University of Agriculture;College of Veterinary Medicine,China Agricultural University;  
【Objective】This study was to investigate the remission effect of lysine on rat jejunal mucosal injury and autophagy.【Methods】The model of heat stress rat was established by constant temperature air table.The change of rat body weight was measured by analytical balance.The morphology of intestinal epithelial cell injury was observed by inverted microscope through HE staining.The expression levels of miRNA-23 band LC3 Bwere detected by RT-PCR.【Result】Heat stress at 40℃for 2 h is the best condition for rats.Feeding with high concentration lysine(1.0%)had the best effect on body weight improvement after heat stress.Through lysine pretreatment and heat stress on rats,the jejunum epithelial injury was relieved significantly.The expression of LC3 Bwas significantly inhibited,while the expression of miRNA-23 bwas significantly increased.【Conclusion】Feeding with high concentration lysine could relieve rat jejunal injury caused by heat stress.The relief was through miR-23 binhibiting autophagy of jejunum epithelial cell caused by heat stress.
【Fund】: 公益性行业(农业)科研专项经费项目(201403051-07)
【CateGory Index】: S852.3
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