Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Beijing University of Agriculture》 2018-03
Add to Favorite Get Latest Update

Cloning and functional verification of McmiR159a in Malus leaves

KANG Yanhui;LUO Rongli;LI yuxin;JI qianlong;YAO Yuncong;Beijing Key Laboratory of New Technology in Agricultural Application/Plant Science and Technology College,Beijing University of Agriculture;  
【Objective】The experimentis to verify the effect of miR159 aon anthocyanin biosynthesis in Malus ornamental crabapple leaves.【Methods】Weselected theleaves of ever-red-leafed ‘Royalty'of Malus ornamental crabapple as experimental material to identify the sequence of miR159 aprecursor gene and topredictitstarget.The McmiR159 aover-expression vector was constructed and transiently transferred into the‘Royalty'tissue culture seedlings.The content of anthocyanin and the expression of structural genes in the pathway of anthocyanin biosynthesis were analyzed by HPLC and qRT-PCR,respectively.【Results】Over expression of McmiR159 ain theleavesof Malus ornamental crabappleincrease the anthocyanin content by downregulating the expression of target and up-regulating the expression of key genes in anthocyanin biosynthesis pathway.【Conclusion】McmiR159 amaypositively effect on anthocyanin synthesis in Malus ornamental crabapple leaves.
【Fund】: 长城学者项目(5045243001)
【CateGory Index】: S685.99
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved