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《Journal of Beijing University of Agriculture》 2018-03
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Molecular cloning and expression of arginine kinase gene of Tetranychus cinnabarinus(Acari:Tetranychidae)

WANG Lei;HUANG Tiran;LI Guangdong;AN Xiangshun;Yang Jin;WANG Xiao;HU Shuai;WANG Pingping;CHEN Qing;Key Laboratory of Urban Agriculture(North China)Ministry of Agriculture,College of Biological Science and Engineering,Beijing University of Agriculture;  
【Objective】This study aims to clone the arginine kinase gene in Tetranychus cinnabarinus,analyze the gene sequence and mRNA expression profiles,and then explore the function of TcAK gene during mite growth and its responses to diapause,thermal stress and UV stress.【Methods】The full-length cDNA encoding arginine kinase from T.cinnabarinus was cloned and identified via RT-PCR and RACE.The putative amino acid sequence was analyzed by bioinformatics methods.The relative expression levels of TcAKin T.cinnabarinus at different developmental stages and in diapausing adult females,as well as under thermal or UV stress conditions,were measured using real-time quantitative PCR.【Results】The results showed that TcAK gene contains an open reading frame of 1071 bp which encoding 356 amino acids and containing typical arginine kinase active site and substrate binding site.During the development of T.cinnabarinus,the expression of TcAK were analyzed.Compared with egg stage,the expression of TcAKincreased at larva and nymph stage,and reached the highest level at the larva stage.And the expression of TcAK decreased at adult stage.In addition,the significant up-regulation of TcAK was observed in T.cinnabarinus in diapausing females,under high/low temperature stress or UV stress.【Conclusion】Arginine kinase may be involved in mite growth and development,and play an important role in T.cinnabarinus against the adverse environment.
【Fund】: 北京市教育委员会科技计划面上项目(KM201410020002);; 北京农学院质量提高经费项目(GJB2015005);; 国家自然科学基金项目(31670648);; 北京市自然科学基金项目(6162004)
【CateGory Index】: S433.7
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