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《Journal of Jilin University(Medicine Edition)》 2009-06
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Antioxidant effect of honeysuckle on hepatic RBL cells of rats in vivo and in vitro and mechanism

GONG Cui-cui1,ZHENG Nai-gang2,3,WU Jing-lan3,LAI Ze-ren1,HEN Pei-xia1(1. Laboratory Center,No.153 Hospital of PLA,Zhengzhou 450042,China; 2. Department of Oncology Basic Sciences,Medical College,Zhengzhou University,Zhengzhou 450001,China;3. Molecular Cell Biology Research Center,Medical College,Zhengzhou University,Zhengzhou 450052,China)  
Objective To explore the antioxidant effect of honeysuckle on hepatic RBL cells of rats in vivo and in vitro and its underlying mechanism,and provide theoretical basis for the application of honeysuckle in clinic.Methods The blood levels of T-AOC,SOD,GSH-Px,GSH or MDA in an antioxidant enzyme system were detected 1-2 h after administration in the established rat models,which were administrated with larger and smaller doses of honeysuckle respectively.The cultured human hepatic RBL cells were exposed to H2O2 as an oxidant and treated with 0.25g·L-1 neysuckle before or after exposure to H2O2.The cell survival and apoptosis were detected by MTT and TUNEL assays.The expression levels of NF-kB,Bcl-2,Bax,HSP-70 and Caspase-3 were determined by immunohistochmistry and Western blotting and the cell anti-oxidant enzyme system was detected by corresponding kits.Results In the antioxidant and anti-apoptotic effects of honeysuckle,the pre-protective effect was markedly higher than the post-protective.The expressions of NF-κB and HSP-70,the expression ratio of Bcl-2/Bax were down-regulated,and the apoptotic rate in pre-protective group was lower than that in post-protective group. Besides,the detected cell level of anti-oxidant enzyme system was up-regulated significantly.In the plasma of rat model the levels of T-AOC,GSH-Px,GSH and SOD were promoted(P0.05),while that of MDA was decreased markedly(P0.05).Conclusion Honeysuckle displays antioxidant capacity through up-regulating the antioxidant enzyme system and down-regulating NF-κB signal transduction pathway.
【Fund】: 河南省科技厅基础与前沿技术研究计划项目资助课题(072300410440)
【CateGory Index】: R285.5
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