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《Journal of Bethune Medical Science》 2014-03
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Effect of bee venom peptide on the proliferation of bladder cancer cells T24

WANG Qiang;LIU Yanru;CHEN Yudong;Department of Urinary Surgery,252 Hospital of PLA;  
Objective To investigate the effect of bee venom peptide on the proliferation and cell cycle of bladder cancer.Methods 0. 1,1. 0,10. 0,100. 0 μg /ml concentrations of bee venom peptide were used to act on the cultivated bladder cancers T24.The propagation supressing measuring method with methyl thiazol tetrazolium( MTT) was applied. Flow cytometry was used to assess the effects of bee venom peptide on the expression of proliferating cell nuclear antigen( PCNA) and cell cycle of T24 bladder cancer cells.Results Bee venom peptide could inhibit proliferation of T24 bladder cancer cells in vitro and inhibit the expression of proliferating cell nuclear antigen dose-dependently( P 0. 05 or P 0. 01). Bee venom peptide could interfere with cell cycle of T24 bladder cancer cells,decrease G2/M phase cells and increase S phase cells. During interference cell cycle,G0/G1of each group was lower than that of the control group( P 0. 05 or P 0. 01),but S phase cells were higher than that of the control group( P 0. 05 or P 0. 01),G2M phase of 10 μg /ml and 100 μg /ml group was higher than that of the control group( P 0. 01). Conclusion Bee venom peptide can inhibite proliferation of T24 bladder cancer cells. The mechanism may be related to inhibition of PCNA expression and interference with cell cycle.
【CateGory Index】: R737.14
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