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《Journal of Beijing Normal University(Natural Science)》 2005-04
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CLONING AND EXPRESSION OF β-AGARASE(AgaA7)

Wang Guanghui~1 Zhang Ming~2 Wei Qun~1( 1)College of Life Science, Beijing Normal University,100875, Beijing, China;2)Jilin Academy of Agricultural Science, 136100, Gongzhuling, Jilin, China)  
In order to study the exprssion product of recombinant β-agarase(AgaA7) gene and its bioactivities, the full β-agarase(AgaA7) sequence is synthesized according to the GeneBank.Then it is inserted into expression vector PQE30. Its sequence is verified and the reading frame is proved to be correct.The recombinant plasmid is transformed into E.coli M15.The molecular mass of the purified expression product is examined by SDS-PAGE and its bioactivities are proved.
【CateGory Index】: Q785
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【Co-references】
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1 Liu Jiang-tao1, Cai Jun-peng1, Wu Bing2 (1College of Food Science and Biotechnology, South China University of Technology, Guangzhou, 510640) (2Centre for Chemical Analysis, South China University of Technology, Guangzhou, 510640);Progress of Studies on Agarase and Its Applications[J];Guangzhou Food Science and Technology;2005-01
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