AN EXPERIMENTAL STUDY ON REVERSIBLE VAS DEFERENS OCCLUSION WITH RING-CLIPS
Zhou Rongxiang, et al (Family Planning Institute, Binzhou Medical College)
In order to study reversible vas deferens with ring-clips, 20 rabbits were divided into four groups: (1) ring-clips with silicon-colloidal nylon line, (2) simple ring-clips, (3) ring-clips with horse-tail, (4) control group. When vas ring-clip vasoligation was performed, silicon-colloidal nylon line or horse tail was put into vas deferens lumen as a support. Sperm were examined for six, twelve months, the sterilization results were good (100％). Ring-clips and support were removed at the six, twelve months after operation. Sperm were examined for another six months, reversible rate was 100％ in group one, 91.67％ in group two and 66.67％in group three. Serum sperm antibody was detected in 66.11％ of the rabbits. Using light and electron microscopes,we found that larger structure and lumen of vas with ring-clips were intact, testis and epididymis were less changed. Histologic bases of reversing this occlusion was revealed in this study. It is suggested that surgical quality, support material and ring-clips pressure influenced the reversal of vas deferens. Sperm antibody, ultrastractral and cytichemical changes of testis and vas were the most important factors that influenced the re-fertilization following reversible vas deferens.