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《Journal of Binzhou Medical College》 1991-01
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Zhou Rongxiang, et al (Family Planning Institute, Binzhou Medical College)  
In order to study reversible vas deferens with ring-clips, 20 rabbits were divided into four groups: (1) ring-clips with silicon-colloidal nylon line, (2) simple ring-clips, (3) ring-clips with horse-tail, (4) control group. When vas ring-clip vasoligation was performed, silicon-colloidal nylon line or horse tail was put into vas deferens lumen as a support. Sperm were examined for six, twelve months, the sterilization results were good (100%). Ring-clips and support were removed at the six, twelve months after operation. Sperm were examined for another six months, reversible rate was 100% in group one, 91.67% in group two and 66.67%in group three. Serum sperm antibody was detected in 66.11% of the rabbits. Using light and electron microscopes,we found that larger structure and lumen of vas with ring-clips were intact, testis and epididymis were less changed. Histologic bases of reversing this occlusion was revealed in this study. It is suggested that surgical quality, support material and ring-clips pressure influenced the reversal of vas deferens. Sperm antibody, ultrastractral and cytichemical changes of testis and vas were the most important factors that influenced the re-fertilization following reversible vas deferens.
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