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《Journal of Binzhou Medical University》 2017-01
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Alteration and clinical significance of CD4~+ CD45RA~+ and CD4~+ CD45RO~+ T cells in metastatic colorectal cancer patients before and after treated with FOLFOX therapy

YU Zeshun;GENG Haitao;SHI Chuanqin;XU Xue;HU Tao;Department of Immunology,Binzhou Medical University;Department of Oncology,Binzhou Medical University Hospital;  
Objective To explore the effect of FOLFOX chemotherapy on the CD4~+primary T cell(CD4~+CD45RA~+)and memory T cell(CD4~+CD45RO~+)in metastatic colorectal cancer(mCRC)patient's peripheral blood and its clinical significance.Methods After using fluorescence antibody anti-CD3,anti-CD4,anti-CD45 RO and anti-CD45 RA to label peripheral blood cells(PBMC)origin from 10 cases of healthy control and 27 cases of mCRC patients before and after FOLFOX chemotherapy,we detected the percentages of CD4~+T cells in PBMC and its subsets CD4~+CD45RO~+and CD4~+CD45RA~+T cells with flow cytometry,and analyzed the difference of these T cell subsets and their relationship with curative effects.Results Compared with healthy people,the ratio of CD4~+T cell in mCRC patient's PBMC before FOLFOX treatment had no difference(P0.05),while the ratio of CD4~+CD45RA~+and CD4~+CD45RO~+T cell in CD4~+T cells decreased(P0.01)and increased(P0.05),respectively.After FOLFOX therapy,both of the CD4~+T celsl and CD4~+CD45RO~+T percentage was higher than that in prechemotherapy(P0.001,P0.05),while CD4~+CD45RA~+T cell was just opposite(P0.001).Meanwhile,in the group of mCRC patients who showed effects for FOLFOX chemotherapy,the percentage of CD4~+CD45RA~+cells in their CD4~+T cells before and after chemotherapy was no difference(P0.05),but significantly higher than that in patients ineffective(P0.01,P0.001),the percentage of CD4~+CD45RO~+T cells was also higher than pre-chemotherapy(P0.05).Conclusion The chemotherapy of FOLFOX regimen not only could increase CD4~+T cells,change the ratio of T cell subsets such as CD4~+CD45RA~+and CD4~+CD45RO~+T cells,but also show more effects to cure the mCRC patients with higher ratio of CD4~+CD45RA~+.
【CateGory Index】: R735.34
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