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《Journal of Binzhou Medical University》 2017-06
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Role of kisspeptin/kiss1r system expressed in the ovary during the process of premature ovarian failure

SHI Hongmei;LIU Ranran;ZHANG Hongqin;School of Basic Medical Sciences,Binzhou Medical University;Department of Radiology,Qilu Hospital of Shandong University(Qingdao);Department of Reproductive Medicine,Yantai Affiliated Hospital of Binzhou Medical University;Department of Histology and Emnryology,School of Basic Medical Sciences,Binzhou Medical University;  
Objective To explore the expression of kisspeptin/kiss1 rsystem in the ovary and the role played during the premature ovarian failure model.Methods Female balb/c mice aged 6 weeks were divided randomly into the normal group,the control group and the POF group.POF mice were treated with Cyclophosphamide(70 mg/kg,suspended in 0.01% DMSO)and the control group received 0.01% DMSO by intraperitoneal injection.One week after the treatment,the serum levels of folliclestimulating hormone(FSH)and estradiol(E2)in three groups were assessed by enzyme-linked immunosorbent assay(ELISA).Ovarian histopathological changes were evaluated by hematoxylin-eosin staining(HE staining).Protein levels of kisspeptin/kiss1 rsystem in the ovary were evaluated by immunohistochemistry.The collagenous fiber was detected by masson method.Results ELISA result showed that compared with those in the control group,levels of E2 decreased and levels of FSH increased markedly in serum of POF mice.The number of growing follicles and the layer of granulosa cells decreased but atresia follicle increased in the ovaries of POF mice.A large amount of collagen fibers had appeared in the follicle in addition to ovarian stroma.Positive cells of kisspeptin/kiss1 rsystem in the secondary follicles decreased significantly in POF mice.ConclusionKisspeptin,expressed in POF mice ovaries,may make follicle reduce the sensitivity to FSH,so that a large number of primary follicles are raised,and eventually the oocyte is exhausted.
【Fund】: 滨州医学院科技计划项目(BY2016KJ36)
【CateGory Index】: R711.75
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