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《Journal of Binzhou Medical University》 2017-06
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Prediction of miR-30b-5p targets gene and its bioinformatics analysis in breast cancer

WANG Shasha;ZHU Jun;SUN Leitao;LIU Weixian;WANG Xiaohong;Department of oncology,Binzhou People's hospital;Department of gastrointestinal surgery,Binzhou Medical University Hospital;Department of neurosurgery,Binzhou Medical University Hospital;Department of Thyroid and Breast Surgery,Binzhou Medical University Hospital;  
Objective To provide theoretical guidance for further research on the role of miR-30 b-5 p in formation and development of breast cancer.Methods The miR-30 b-5 p in tissue chip by ISH was detected.The expression of miR-30 b-5 p in breast cancer cell lines was compared by fluorescence quantitative PCR.The target genes of miR-30 b-5 p were forecast.KEGG and GO enrichment analyses were carried on.Results MiR-30 b-5 p was down-regulated in breast cancer tissues,with statistical difference compared to that in adjacent tissues(P0.01);the expression levels of miR-30 b-5 p were found to be significantly associated with tumor node metastasis(TNM)staging and distant metastasis(P0.01),without association with age and lymphatic metastasis.The comparison of expressions of miR-30 b-5 p in MCF-7,MDA-MB-231,MDA-MB-415,MDA-MB-453 and MDAMB-468 breast cancer cell lines showed that expressions in comparative normal breast tissues were low and the MDA-MB-231 cells had the lowest expression(P0.01).The analysis of KEGG and GO indicated that miR-30 b-5 p was involved in pathways in cancer,P53 signaling pathway,Wnt signaling pathway and cell cycle and played a role in cell metabolism regulation,gene expression regulation,RNA metabolism and cellular biological function.Conclusion The expression of miR-30 b-5 p is down-regulated in breast cancer.It may play an important role in breast cancer cell metabolism,gene expression and so on through pathways in cancer,P53 signaling pathway,Wnt signaling pathway and Cell cycle.
【Fund】: 山东省自然科学基金(ZR2017LH072);; 滨州市科技发展计划项目(2015ZC0301);; 滨州医学院科研计划与科研启动基金(BY2014KYQD36)
【CateGory Index】: R737.9
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