Spectroscopic Studies on the Interaction between PBBHAMF and Calf Thymus DNA
WANG Rui-ling;XU Li-fan;DENG Cheng-yu;HUANG Xiao-yan;HUANG Zhen-zhong;College of Chemistry and Chemical Engineering,Jiangxi Normal University;
The interaction between 4'-phenyl-3-bromo-8-[N,N-bis( 2-hydroxyethyl) aminomethyl] flavone( PBBHAMF) and calf thymus DNA( ct-DNA) has been studied. PBBHAMF could quench the fluorescence of the AO-DNA complex with the use of acridine orange( AO) as a fluorescent probe,the quenching mechanism is mainly a static quenching procedure. When bound to DNA,PBBHAMF shows hypochromism in absorption spectra,that means intercalation of PBBHAMF into DNA. Moreover,the increase in relative viscosity and the CD spectral change in 274 nm also support that the major binding mode is an inserting interaction. The FT-IR spectroscopy demonstrates that electrostatic attraction exists between PBBHAMF and DNA simultaneously.