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《Acta Agrestia Sinica》 2017-04
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Effect of Different Degrees of Rocky Desertification on Genetic Diversity of Soil Bacteria In Shrubby Grassland

CHEN Ying;WANG Qian;WANG Zi-yuan;WANG Xiao-li;Guizhou Institute of Prataculture;  
This study was carried out to evaluate the effect of rocky desertification on genetic diversity of shrubby grassland soil bacteria.Soil samples of four different degrees of rocky desertification shrubby grasslands were collected in the restoration and treatment point of Puding County,Guizhou Province,and soil sample of corn planting sites in rock cracks was also collected as control.The treatments were as follows:potential(A),slight(B),medium(C),severe rocky desertification(D)and the control(E).The soil bacterial community structure was studied using denatured gradient gel electrophoresis(DGGE)analysis.The results showed that the complexity and richness of bacterial communities showed a declining trend with the aggravation of desertification,and the Shannon-Wiener and richness index of the soil sample A were higher than that of B,C,D.But the ShannonRocky desertification;Shrubby grasslands;Soil bacteria;Genetic diversityWiener,Evenness and Richness index of the control(E)were the lowest.It showed that the complexity and richness of soil bacteria significantly decreased after soil cultivation.Clustering analysis showed that they were divided into two clusters in the phylogenetic tree:the one cluster was the control(E)and the others were A,B,C,D.Among them,A and B had high degree of similarity,and the C and D had the maximum similarity.The phylogenetic analyses showed that the 13 sequenced fragments were clustered into 10 branches,and the soil bacteria in different degrees of rocky desertification shrubby grasslands soil had different community structures and had high degree of genetic diversity.
【Fund】: 贵州省科学技术基金(黔科合J字[2012]2198);; 贵州省联合基金(黔科合LH字[2014]7699)资助
【CateGory Index】: S812.2
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