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《Acta Agrestia Sinica》 2017-04
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Physiological Characteristic Diversity in Reversed and Non-Reversed Spike Buds of Switchgrass

WANG Yong-feng;LI Mao;CUI Gui-bin;XU Kai-Jie;SUN Feng-li;ZHANG Chao;LIU Shu-dong;XI Ya-jun;College of Agronomy,Northwest A&F University;Institute of Cotton Research of Chinese Academy of Agricultural Sciences;  
Swithchgrass(Panicum virgatum L.)is a short-day plant.Due to its wide adaptability and high biomass yield,switchgrass was regarded as the model biofuel plant world widely.Previous study on switchgrass showed that the shoot apexes of tillers at elongation stage 4,which harboring a spike 1~2 cm long,incubated on shoot induction medium would induce flower reversion and finally formed massive regeneration buds,named as reversed spike buds.Correspondingly,shoot apexes incubated on the MS basal medium showed no flower reverse and finally formed little buds,named non-reversed spike buds.To discover the underlying mechanism of the reversed spike bud formation,some physiological indexes of the reversed spike buds and non-reversed spike buds were compared.The results showed that the reversed spike buds had higher sugar content but lower chlorophyll content,and they were more effective in nitrogen utilization;the activity of antioxidant enzyme system(SOD,POD,CAT,PPO)were higher;the exogenous supplementary of cytokinin exhibited little effect on endogenous cytokinin zeatin,however it suppressed the endogenous indoleacetic acid to some extent,which might play an important role in spike buds initiation.Abscisic acid affected spike buds development at the late stage while gibberellin affected spike buds development throughout the whole process.
【Fund】: 国家自然科学基金(31171607 31371690)资助
【CateGory Index】: S578
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