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《Acta Agrestia Sinica》 2017-04
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Introduction Of OsVTE Gene Enhanced Osmotic Stress Tolerance of Transgenic Chicory Plants

MU Zhi-xin;SHI Ying;ZHANG Li-jun;ZHOU Jian-ping;Institute of Crop Germplasm Resources Research,Shanxi Academy of Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,P.R.China,Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops;  
Plant genetic engineering was widely used to improve crop viability and tolerance to environmental stresses.In this report,an OsVTEgene was introduced into Cichorium intybus L.‘Puna'by Agrobacterium-mediation transformtion.Transgenic chicory plants were obtained under Kanamycin selection,and confirmed by PCR and Southern-blot tests.Effects of different concentrations of sucroses,agarose and mannitol were used as a mimic of drought stress codiction to investigate drought tolerance of chicory seedlings.The results showed that drought tolerance of transgenic plants was better than those non-transgenic ones.Activity of peroxidase(POD)and superocide(SOD)dismutase of transgenic seedings at the sucrose concentration of 50 g·L~(-1),agarose concentration of 10 g·L~(-1) and mannitol concentration of 30 g·L~(-1) was 2~3 times higher than that of non-transgenic ones.Malonaldehyde(MDA)content in transgenic seedlings was lower than that of non-transgenci ones.Overall,stable transgenic plants with OsVTE were obtained.The transgenic plants showed higher drought tolerance,content of POD and SOD dismutase in transgenic plants was increased under drought stress conditions.
【Fund】: 国家自然科学基金(31501372);; 山西省农科院所长青年引导专项(yydzx02);; 山西省农科院种业发展项目(2016zyzx41)资助
【CateGory Index】: S548
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