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《Journal of Chengdu Medical College》 1997-02
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SIMUTANEOUS DETERMINATION OF RETINOL AND α-TOCOPHEROL IN SERUM BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Ma Shuqing Department of Clinical Laboratory,Affiliated Hospital  
A method was described for determining retinol and α—tocopherol simutaneously in serum.by high-performance liquid chromatography with tocopherol acetate as internal standard.The relative retention time of retinol,α—tocopherol and tocopherol actate was 2.60,5.66 and 7.14min respectively.The analytical recovery of this method was 97.2% for retinol and 95.6% for α—tocopherol.The within-run CVs were 2.0~4.2% for retinol.(x=1.41~3.99μmol/L)and 0.4~2.4% for α—tocopherol (=15.21~61.66umol/L).between-run CV was 5.3% for retinol(=1.5oμmol/L)and 3.9% for α—tocopherol(=15.53μmol/L).The method was linear between 0.44 to 6. 98μmol/L for retinol and between 3.86 to 61.66μmol/L for α—tocopherol.The concentrations of seurm retinal determined by the method were 2.33±0.58μmol/L in males and 162±0.38μmol/L in females;and those of α—tocopherol were 16.32± 4.81μmol/L in males and 14.70±4.27μmol/L in females.
【CateGory Index】: R115
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