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《JOURNAL OF CHENGDE MEDICAL COLLEGE》 2000-03
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THE EFFECTS OF TEA PIGMENTS ON EXPRESSION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE FROM CULTURED HUMAN UMBILICAL VASCULAR ENDOTHELIAL CELLS

HT5SS][KH2D][WT5BZ]XUAN Wan li 1,WANG Shen wu 2,HAN Chi 3,CHEN Jun shi 3 [WT5BX](1.Institute of Materia Medica,chengde Medical College,Hebei Chengde 067000,China; 2.Peoples Hospital of Beijing Medical University; 3.Institute  
Objective:To study the effects of tea pigments on the expression of endothelial nitric oxide synthase(eNOS) from cul tured human umbilical vascular endothelial cells in vitro.Methods:The endothelial cells were exposed to different doses of tea pigments and incubated at 37℃ for 24 hours.The expression of endothelial nitric oxide synthase was detected by using western blot and reverse transcription polymerase chain reaction(RT PCR).Results:The results showed that tea pigments could stimulate expression of eNOS in the mRNA and protein level with significant dose effect relationship(P0.05).Conclusion:The results suggest that the effect of tea pigments on eNOS expression is one of the mechanisms of its antiatheroselerosis.
【CateGory Index】: R543.1
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