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《Journal of Chengde Medical College》 2000-03
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SUBSTITUING SPLENIC CELLS OF MICE FORHUMAN LYMPHOCYTES IN THE ROSETTE ASSAY FOR EXPERIMENTAL TEACHING

KH2D][WT5BZ]DENG Shu hua,et al [WT5BX](Department of Micrology and Immunology,Chengde Medical College, Hebei Chengde 067000,China)  
Objective:To investigate the possibility of the E rosette assay in that human lymphocytes could be replaced by splenic cells derived from mice for experimental teaching.Methods:Mice were immunized with 0.4ml of SRBC(5%),prepared the suspension of splenic cells after 4 and 21 days,and then made the E rosette assay.Results:At 4 and 21 day after immunized with SRBC,there was no significance at the percentage of specific E rosette forming cells among the splenic cells mixed with SRBC for 10 or 120minutes(P0.05).They were from 28.64±4.48% to 34.38±7.25%.The morphology,size and the pattern of staining of mice splenic cells were similar to that of human T lymphocytes.Conclusion:Substituting mice splenic cells for human lymphocytes in the E rosette assay can be used in the students experimental teaching.The result may also show the function of specific humoral and cellular immunity in experimental animals.
【CateGory Index】: R446.6
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