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《Journal of Chengde Medical College》 2010-01
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GONG Ming-yu,LIU Yong-ping,LI Fang,et al (Department of Biochemistry,Chengde Medical College,Hebei Chengde 067000,China)  
Objective:To investigate protective effects of breviscapine pretreatment on myocardial cells apoptosis of ischemia/reperfusion injury rats.Methods:40 SD rats were divided into sham operation group,model group and 2 breviscapine pretreatment groups at random (n=10).The rats in breviscapine pretreatment groups were intraperitoneally injected 25 mg/kg/d and 50 mg/kg/d breviscapine respectively 1 week prior to ischemia/reperfusion;while the rats in other two groups were given NS instead.Myocardial ischemia/reperfusion injury model was established by ligating the anterior interventricular branch of the left coronary artery:ligation 30 minutes and reperfusion 2 hours.The AnnexinⅤ-EGFP/PI staining and flow cytometry were used to detect the apoptosis of myocardial cells.Results:Breviscapine pretreatment could decrease apoptosis ratio of myocardial cells of ischemia /reperfusion injury rats obviously (P0.05,P0.01).Conclusions:Breviscapine pretreatment can protect the myocardial cells of ischemia/reperfusion injury rats by inhibiting apoptosis of myocardial cells.
【CateGory Index】: R285
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