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《Journal of Chengde Medical College》 2010-01
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LIANG Wen,PAN Hai-feng,ZHANG Xiao-feng (Institute of Chinese Mateia Medica,Chengde Medical College,Hebei Chengde,067000)  
Objective:To establish the method for determination Paeoniflorin content in Jianpi Huashi Grains by HPLC.Methods:Discovery C18 column (4.6mm×250mm×5μm) was used:the mobile phase was methanol:0.1% formica acid (23:77),detection wavelength was 230 nm and the column temperture was 25℃.Results:There had a good linear correlationship at the range 1.35-6.75μg,the average recovery was 99.35%,RSD was 1.33%.Conclusions:The method that determining Paeoniflorin content in Jianpi Huashi Grains by HPLC is convenient,reliable,accurate and can be acted as the quality control of white peony root in Jianpi Huashi Grains.
【CateGory Index】: R286
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