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《Journal of Chengde Medical College》 2014-01
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INHIBITING EFFECTS OF SIRNA TRANSFECTION CONCENTRATION AND TIME ON ANXA5 EXPRESSION IN HELA CELLS

WANG Xiao-jie;WANG Yong-wei;QU Yin-e;Basic Medical Sciences of Hebei United University;  
Objective: To optimize the conditions of siRNA targeting ANXA5 transfected into HeLa cells,so to provide bases for further studying effects of ANXA5 low expression on biological functions of cervical cancer cells. Methods:siRNA targeting ANXA5 was transfected into HeLa cells,with different ratios of ANXA5 siRNA and liposomes,and different transfection time. Western Blot was used to detect the ANXA5 protein expression in HeLa cells. Results:The ANXA5 protein expression in HeLa cells was the lowest(the inhibitory effects was the strongest) when the ratio of siRNA and liposomes was 0.4:1 and transfection time was 72h. Conclusions:The ratio of siRNA and liposomes and transfection time can infl uence the expression of ANXA5 in HeLa cells,and has the best ratio and transfection time.
【Fund】: 河北省科学技术研究与发展计划项目(10276142)
【CateGory Index】: R737.33;Q78
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 LI Xin GAO Fu-lu (Chengde Medical College,Hebei Chengde 067000,China);EXPRESSION OFANXA5 IN UTERINE CERVICAL CARCINOMA CELL LINE HELA AND SIHA CELLS[J];Journal of Chengde Medical College;2008-01
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