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《Journal of Chengde Medical College》 2014-01
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DETERMINATION OF BERBERINE HYDROCHLORIDE CONTENT IN ZHIZIJINHUA DISPERSIBLE TABLET BY HPLC

CHEN Wei;ZHAO Dong-feng;SHI Hong-juan;Hebei Key Laboratory of Study and Exploitation of Traditional Chinese Medicine,Institute of Chinese Materia Medica;  
Objective:To establish a method for determining of berberine hydrochloride content in ZhiziJinhua Dispersible Tablet.Methods:HPLC was used to determine berberine hydrochloride content in ZhiziJinhua Dispersible Tablet. The chromatographic column was Diamonsil-C18(4.6×150mm,5μm),with acetonitrile-0.6% NaH2PO4(30: 70) as mobile phase,fl ow rate: 1.0ml/min,detection wavelength: 350nm. Results:The linear range of berberine hydrochloride was 2.5-50μg(r=0.9995); the average recovery of berberine hydrochloride was 100.85%,RSD was3.7%(n=6). The average content of berberine hydrochloride in ZhiziJinhua Dispersible Tablet was 0.633%.Conclusions:Determination of berberine hydrochloride content in ZhiziJinhua Dispersible Tablet by HPLC in this study is simple and reproducible,and can be used for determining berberine hydrochloride content in ZhiziJinhua Dispersible Tablet.
【Fund】: 承德市科学技术研究与发展计划项目(201021020)
【CateGory Index】: R284.1
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