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《Journal of Chengde Medical College》 2018-01
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COMPARATIVE STUDY ON FINGER-PRINT AND TOTAL FLAVONOIDS CONTENT IN FOUR DIFFERENT EXTRACTION PARTS OF HAWTHORN LEAVES

GAO Jing;LI Ming-chen;XU Bao-xin;Institute of Chinese Materia Medica/Hebei Key Laboratory of Research and Exploitation of Traditional Chinese Medicine,Chengde Medical College;  
Objective: To comparatively analyze 4 different extraction parts(A.50% ethanol extract from hawthorn leaf,B.alcohol extract of hawthorn leaf after macroporous resin,C.ethyl acetate part of hawthorn leaf,D.n-butanol part of hawthorn leaf). of hawthorn leaves by establishing HPLC finger-print and detecting total flavonoids content. Methods:The fingerprint of hawthorn leaves was established by HPLC method using Diamonsil C18(4.6 mm×250 mm, 5 μm) chromatographic column, acetonitrile-0.1% acetic acid-tetrahydrofuran as mobile phase with gradient elution, detection wavelength 320 nm, flow rate of 0.9 ml/min, and the column temperature was 30℃. The total flavonoids content was detected by ultraviolet spectrophotometry. Results: The common peaks of 4 extraction parts of hawthorn leaves in types and numbers had differences; The differences between extract C and the other three extracts was the largest. The total flavonoids content in extract B was the highest. Conclusions: This study can lay foundation for further development and utilization of hawthorn leaves extracts, and also can provide references for studying pharmacodynamic differences of different extraction parts of hawthorn leaves extracts.
【Fund】: 河北省中医药管理局科研计划项目(2017216)
【CateGory Index】: R284.1
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