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《Journal of Chongqing University of Technology(Natural Science)》 2017-08
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Identification and Characterization of Bacterium with Carbonic Anhydrase in Karst Landform

YI Zhe;YE Jiangyu;LI Darong;DOU Jianjun;SHI Yuzhu;Faculty of Urban Construction and Environmental Engineering,Chongqing University;Chongqing Rongji Environmental Technology Co.,Ltd.;  
Carbonic anhydrase(CA) which can catalyze inter-conversion between CO_2 and HCO_3~- rapidly exists widely in prokaryote cells,and the unique conditions of Karst landform is very suitable for the expression of microbial carbonic anhydrase. In this paper,the soil samples were collected from the Jinfoshan scenic spot with the typical Karst landform in Chongqing,isolating bacterium with strains high-yielding carbonic anhydrase. A strain with the highest enzyme activity was selected to perform gram stain microscopy and evaluate the stationary phase. Getting the species of the strain by the identification result of 16 S rRNA. Then,it explores the influence of the main environmental factors(such as temperature,pH,mental ions and anions),on the activity of it 's carbonic anhydrase. It turned out that the bacterial strains C8 is a gram positive bacterium,which has a short pole shape and reaches the stable stage in 18 hours. And the identification result of 16 S rRNA is the Bacillus sp. The carbonic anhydrase activity of the Bacillus sp have good thermal stability. Neutral and alkaline of the environment was fit for keeping stability of CA. A certain range of concentration of mental ions such as Ca~(2+),Mg~(2+),Zn~(2+)and Fe~(2+)along with anions such as SO_4~(2-) and Cl~- have few effects on the CA activity of B. RA51. This research provides scientific basis for applying the B.RA51's CA in circulating cooling water.
【Fund】: 国家科技重大专项(2013ZX07104004)
【CateGory Index】: Q93
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