Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Fisheries Science》 2018-04
Add to Favorite Get Latest Update

Functional Genes of Heterotrophic Nitrification and Aerobic Denitrification in Bacterium Pseudomon asaeruginosa

LIU Xing;LI Lianxing;BO Xianglan;CHEN Jichu;ZHOU Shengjie;WANG Jun;WANG Lihong;CHEN Chengxun;Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University;Tianjin Lida Water Resources Development Co.,Ltd;The Key Laboratory of Microbial Preparation Enterprise in Tianjin,Dingzheng Animal Pharmaceutical Co.,Ltd;  
The functional genes including amoA,napA,narG,nirK,nirS,cnorB,qnorB,nosZ,and nifH were amplified,and sequenced by restriction enzyme digestion and PCR and the target gene fragment was analyzed by agarose gel electrophoresis in order to evaluate the genes of denitrification and nitrification function and to understand denitrification process of Pseudomonas aeruginosa YY24.The results showed that amoA gene is the coding gene of ammonia monooxygenase in YY24.There was membrane-bound nitrate reductase as cytochrome cd1 reductase nirS and nosZ gene in YY24.It was found that nitric oxide reductase catalyzed NO to N2 O and had gene qnorB in YY24.
【Fund】: 国家自然科学基金(面上)资助项目(31270456);; 天津市水产产业技术体系创新团队项目(ITTFRS2017001);; 天津市科技支撑计划重大项目(12ZCDZNC05900);; 天津市应用基础与前沿技术研究计划(重点项目)(13JCZDJC29200);; 天津市教委一般项目(20140626)
【CateGory Index】: S917.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved