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《Fisheries Science》 2018-04
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Isolation,Identification and Drug Sensitivity of Pathogen Plesiomonas shigelloidesin Burbot Lota lota

ZHANG Xiaoping;YANG Xing;LI Xiaoyi;LI Zhengyou;SHANG Baodi;Guizhou Fisheries Research Institute;  
In the middle of 2014,a disease was found in burbot Lota lotain a farm in Guizhou province,the diseased burbot showing emaciated,anorexia,and black skin;swimming alone;hemorrhagic jaw,liver and abdomen;rot fin;kidney with hemorrhagic spots;empty intestine;some diseased fish having damaged back.Bacteria were isolated from the liver and kidney of infected burbot,and identified as Plesiomonas shigelloides by morphological observation,physiological-biochemical characteristics and 16 S rDNA sequence analysis.The pathogenicity test and drug susceptibility were conducted in the diseased burbot challenged with the isolated bacterium.The pathogenic bacterial strain had LD_(50) of 4.3×10~7 cfu/mL and was sensitive to ofloxacin,enrofloxacin,norfloxacin and enoxacin,resistant to piperacillin,clarithromycin,tobramycin and gentamicin.The findings indicate that the burbot disease is caused by P.shigelloides to provide theoretical basis and reference with the prevention and treatment of the disease.
【Fund】: 贵州省特色水产产业技术体系项目(GZCYTX2017-01102);; 贵州省科技厅农业攻关项目(黔科合NY[2015]3003-1号)
【CateGory Index】: S943
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