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《Journal of Yangtze University(Natural Science Edition)》 2011-03
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Construction and Identification of Recombinant Shuttle-plasmid with pbp39 from Brucella

ZHONG Bei, YANG Yu-ying College of Animal Science, Yangtze University, Jingzhou, Hubei 434025, China ZHANG Xi-chen College of Stockbreeding and Veterinary,Jilin University,Changchun,Jilin 130062,China GU Yu-fang, CHEN Zhen-wei, DUO Ting College of Animal Science, Yangtze University, Jingzhou, Hubei 434025, China  
In order to construct the E.coli-Mycobacterium shuttle vector expressing Periplasmic binding protein (PBP39) and P276. pbp39 sequence and p276 gene were amplified from the genome of Brucella and plasmid of PMD 18-T- p276 by PCR respectively. pbp39 gene was cloned in E.coli-BCG shuttle-plasmid pMV361 to get pMV361-pbp39. Then a new recombinant plasmid pMV361-pbp39-p276 was constructed by inserting p276 into pMV361-pbp39. The results showed the cloned genes pbp39 sequence and p276 were correctly inserted into the vector pMV361, which was confirmed by PCR amplification of pMV361-pbp39-p276. pMV361-pbp39-p276 was expected to secretively express protective antigen pbp39-p276 of brucella in BCG(Bacille Calmette-guerin). It provides the possibility of further researches on the development of new anti-brucella vaccine.
【CateGory Index】: Q784
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