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《Chinese Journal of Neuromedicine》 2007-11
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Experimental study on the activation of ERK1/2 signaling pathway in diffuse brain injury of rats

LI Jin-xing ZHAO Hai-mei ZHU Xian-li LI Yu WANG Chong ZHAO Jia-shan.Department of Neurosurgery,the Third Affiliated Hospital of Nanchang University(the First Hospital of Nanchang),Nanchang 330008,China  
Objective To investigate the temporal and spatial changes of the activation of extracellular signal-regulated kinase1/2(ERK1/2)signal transduction pathway in diffuse brain injury (DBI)of rats by determining the expression of phosphorylated ERK1/2(pERK1/2),a key kinase of ERK1/2 cascade.Methods The Marmarou's free fall injury method was applied to induce DBI in rat models,Western blot assay was used to detect the expression ofpERK1/2 protein in injured brain tissues of rats and immunohistochemical techniques were applied to detect the regional distribution of pERK1/2-positive cells in injured brain tissues.Results Immunohistochemistry demonstrated that the pERK1/2 expression significantly increased after DBI in brain tissues of rats,reached its peak as early as 5 rain,later decreased immediately,rebounded during 12 h and 24 h,reaching a second peak during this period,and lasted the high-level expression until 72 h.The expression of pERK1/2 was localized mainly in cytoplasm during 30 min and 3 h after DBI and at 24 h after DBI,in nucleus and cytoplasm,but mainly in nucleus.The positive immunostaining cells of pERK1/2 were predominantly observed in the deep cortex,secondly in the hippocampus and thirdly in the ependyma and choroid plexus.Conclusion ERK1/2 pathway was rapidly and lastingly activated following DBI in rats;the positive immunostaining cells of pERK1/2 were predominantly observed in the deep cortex,though also observed in the hippocampus,ependyma and choroid plexus.
【CateGory Index】: R651.15
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1 Liu Liying1,Huang Chen1,3,Li Zongfang2 et al1Key Laboratory of Environment and Genes Related to Diseases,Ministry of Education,The Center Laboratory for Biomedical Research,Xi'an Jiaotong University College of Medicine,Xi'an 7100612Department of General Surgery,the Second Hospital,Xi'an Jiaotong University College of Medicine,Xi'an 7100043Department of Genetics and Molecular Biology,Xi'an Jiaotong University College of Medicine,Xi'an 710061;Related Genes for HeLa Cell Apoptosis Induced by siRNA-mediated MAPK p42 Silencing[J];Acta Medicinae Universitatis Scientiae et Technologiae Huazhong;2008-01
2 SHENG Wei,WANG Kang,PING Gao-feng,LI Xu-qi,WEI Guang-bing,HUANG Chen.Department of General Surgery,The First Affiliated Hospital of Medical College,Xi'an Jiaotong University,Xi'an 710061,China;Effects of Inhibit Extracellular-Signal Regulated Kinase 1/2 Pathway on Apoptosis of Hepatoma Carcinoma Cell SMMC-7721[J];Chinese Journal of Bases and Clinics in General Surgery;2007-06
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