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《Chinese Journal of Neuromedicine》 2007-11
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The expression of Nip3 and its relationship with neuron apoptosis in the cerebral cortex of mice following chronic hypoxia/reoxygenation

CAI Kai-jin ZENG Yi-ming QIU Jian-long.Emergency Department,the Second Clinical College,Fujian Medical University,Quartzhou 362000,China  
Objective To evaluate the impact of chronic hypoxia/reoxygenation on the Nip3 expression and neuron apoptosis in the cerebral cortex of mice,and explore the possible mechanism of damage to the brain by sleep apnea syndrome(SAS).Methods The establishment of mouse models of chronic hypoxia/reoxygenation:Male ICR mice were placed in a chamber,where the oxygen concentration changed periodically from(21.72±0.55)% to(6.84±0.47)% every 2 min, hypoxia/reoxygenation 8 h per day.The 30 male ICR mice were randomly divided into four groups: control group(n=10)and hypoxia/reoxygenation groups(n=20),which consisted of hypoxia/reoxygenation 2-week group(n=5),4-week group(n=5)and 8-week group(n=10). Immunohistochemical techniques and terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end-labeling(TUNEL)assay were respectively used to detect the expression of Nip3 in the frontal lobe cortex of mice and neuron apoptosis.Results The expression of Nip3 and number ofapoptotic neural cells in the cerebral cortex of hypoxia/reoxygenation groups were all increased,compared with that of the control group(P0.05)and that in the 8-week and 4-week groups were also higher than that in the 2-week group(P0.05);the comparison between the 8-week and 4-week groups had no statistical significance(P05).The expression of Nip3 was positively correlated with neuron apoptosis in the cerebral cortex(r=0.901,P0.05).Conclusion Chronic hypoxia/reoxygenation may enhance the expression of Nip3 in the cerebral cortex and initiate apoptosis of cortical neurons,and thus,it might be one of the mechanisms for the damage to the nervous system.
【CateGory Index】: R766;R741
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