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《Chinese Journal of Neuromedicine》 2007-11
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Expressions of COX-2 and mPGES-1 in carotid atherosclerotic plaques of rabbits and effects of celecoxib on their expressions

LIU Heng-fang YANG Qi-dong MIAO Wang.Department of Neurology,Fifth Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China  
Objective To explore the expressions ofcyclooxygenase type 2(COX-2)and membrane-associated prostaglandin E2 synthase-1(mPGES-1)in the carotid atherosclerotic plaques of rabbits and effects of celecoxib on their expressions.Methods Thirty-two male New Zealand white rabbits were assigned into the normal control group(n=8,group A)and animal model groups(n=24). After the positioning of improved silicone rubber collars around the right carotid arteries to induce intimal thickening,24 rabbits were fed with 1% high cholesterol diet for 14 d to establish rabbit models with carotid atherosclerotic stenosis.The rabbit models were randomly divided into non-intervened carotid stenosis group(group B),small-dose celecoxib treatment group(15 mg/kg,group C)and large-dose celecoxib treatment group(35 mg/kg,group D),n=8 in each group.Four weeks later alter treatment, bilateral carotid arteries were dissected into two equal sections after the rabbits were put to death,and the expressions of COX-2 and mPGES-1 in carotid plaques after intervention were respectively determined by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)and Western blot assay. Results The expressions of COX-2 and mPGES-1 in carotid atherosclerotic plaques of rabbit models in group B,C,D were significantly higher than that in group A and the difference was significant(P0.05); the expressions of COX-2 and mPGES-1 in carotid atherosclerotic plaques in group C and D were significantly lower that that in group B(P0.05).Conclusion Celecoxib may inhibit the expressions of COX-2 and mPGES-1,and interfere with the inflammatory response which plays the key role in the pathological progress of carotid atherosclerotic plaques,and thus slow the progress of carotid atherosclerosis.
【CateGory Index】: R743
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