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《Journal of Chongqing Normal University(Natural Science)》 2018-04
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Cloning,Bioinformatic,and Expression Analysis of Spo11 Gene of Crucian Carp in Caohai Lake of Guizhou Province

DONG Ranran;CHEN Xiuyun;WANG Gangyi;Lü Changqian;College of Animal Science,Guizhou University;  
[Purposes]To explore the effect of environmental hormone Nonylphenol(NP)on the Spo11 gene expression of the crucian carp(Carassius auratus)in Caohai lake of Guizhou Province.[Methods]The cDNA of Spo11 gene was cloned by PCR in crucian carp.And its physico-chemical properties and structure characteristics were analyzed by bioinformatics.Spo11 gene expression were detected after the fish were treated with NP mass concentrations of 0,25,50,and 100μg·L~(-1) for 28 days.[Findings]It was shown that the sequence of Spo11 gene was 1 471 bp(GenBank ID:MF66470)in length,containing 66 bp 5′UTR,1 152 bp ORF,and 253 bp 3′UTR.The predicted molecular weight was 43.23 kDa,and the value of PI was 6.89.Proteins are mainly made up of leucine,serine and valine.And the protein is an unstable hydrophilic protein containing signal peptide.Its secondary structures were shown to be mainlyα-helix and random coil.Compared with the control group,the relative expression of Spo11 gene were significantly higher in medium and high concentration groups after NP treatment(p0.05).[Conclusions]It is shown that the environmental endocrine disruptor nonylphenol can affect the Spo11 gene expression of crucian carp in Caohai lake of Guizhou Province.
【Fund】: 贵州省科技厅联合基金(黔科合LH字[2015]7678);; 教育部重点实验室开放课题(No.FFRD-2015-01)
【CateGory Index】: S917.4
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