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《Journal of Chongqing Normal University(Natural Science)》 2018-04
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Cadmium Induces Histological Damage and Oxidative Stress in the Liver of Zebrafish

XIE Dongmei;GONG Shiling;LI Yingwen;CHEN Qiliang;Chongqing Key Laboratory of Animal Biology,College of Life Science,Chongqing Normal University;  
[Purposes]To assess the effects of cadmium exposure on the histology and antioxidant status in the liver of zebrafish(Danio rerio).[Methods]Adult male and female zebrafish were exposed to 0(control),5(low concentration group),and 25μg·L~(-1)(high concentration group)Cd2+for 30 days,and then livers were sampled for histology examination and for analysis of the activity of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPX)and glutathione S-transferase(GST),the content of glutathione(GSH)and malondialdehyde(MDA)as well as the expression levels of antioxidant enzyme-related genes(sod1,cat1 and gstr).[Findings]Cd2+exposure resulted in vacuolization and nuclear pyknosis of hepatocytes in both sexes.In males,the activities of SOD,CAT and GPX,the contents of GSH and MDA,and the mRNA level of cat1 in high concentration group were significantly higher than those in the control group,but in low concentration group,only MDA content was significantly increased.In females,the activities of SOD and CAT,the content of MDA and the expression levels of sod1 and cat1 in low concentration group were significantly higher than those in the control group,while GSH content and gstr mRNA level in high concentration group were lower than those in the control group.[Conclusions]Cd2+exposure can induce histological damage and oxidative stress in zebrafish liver,exhibiting gender-differences responses.
【Fund】: 重庆市基础与前沿研究计划项目(No.cstc2015jcyjA80012);; 重庆师范大学校级基金项目(No.15XLB012);重庆师范大学研究生科研创新项目(No.YKC17009)
【CateGory Index】: X171.5
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