Effects of Vanadate on Renal Function in Diabetic Rats
Ai Zhihua, Zhang Zhonghui.( Department of Endocrinology, Southwest Hospitol,The Thind Military Medical University, Chongqing 400038)
Objective: To study the effects of Vanadate(Va) on renal function,and structure in streptozotocin induced diabetic rats and to provide the experimental bases for clinical application of Va. Methods: Sodium orthovasnadate was administered to normal and diabetic rats. 24h urinary protein excretion(UPE) and clearance of creatinine(Ccr) were determined in normal rats and diabetic rats at 12nd week,and the kidaneys were processed for morphological examination with light and eletron microscopes. Mean glomerular plane area (MGPA) and mean glomerular volume(MGV) were calculated. Results: Ccr.LKW.MGPA .and MGV increased at 12nd week in diabetic rats. The electron microscopic results showed that the ultrastructures of kidney were significanty deranged in diabetic rats. Ccr.LKW.MGP and MGV in Vatreated diabetic rats decreased to approach normal level,and the pathologic changes of kidney were improved significantly. In Va-treated noraml rats,no changes were found in renal function. No increase of plasma insulin was found. Conclusion: Va could contribute to the improvement of diabetic nephropathy in diabetic rats.