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《Chongqing Medicine》 2016-03
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Establishment renal calculus rat model induced by calcifying nanoparticles

Su Hongwei;Wang Jie;Zhu Yongsheng;Deng Qingfu;Pei Lijun;Wang Juan;Department of Urology,the Affiliated T.C.M Hospital of Luzhou Medical College;Department of Urology,the Affiliated Hospital of Luzhou Medical College;  
Objective To establish renal calculus rat model induced by calcifying nanoparticles.Methods We extracted and cultivated calcifying nanoparticles from clinically diagnosed patients with nephrolithiasis.Forty adult male SD rats were divided into4groups(n=10each):group A(given an intravenous injection of calcifying nanoparticles),group B(given an intravenous injection of calcifying nanoparticles and taken Shenshitong granules),group C(given an intravenous injection of calcifying nanoparticles and intraperitoneal injection of gallium nitrate),group D(as normal control).Eight weeks later,all rats were sacrificed.The kidneys were examined for pathology.Results Histopathological studies revealed calcifying nanoparticles induced renal tubular epithelial cells fatty degeneration and renal tubular crystallization,and caused renal interstitial infiltration with inflammatory cells.The formation of renal crystals in group A was more obvious than those in group B and C(P0.05).In group D,no crystals were formed and no pathological changes occurred.The levels of urinary calcium and urinary creatinine(of All group)at one day before the observation period(T_2)were higher than those at 4weeks after injection(T_1)(P0.01).The levels of urinary calcium and serum creatinine in group A and B and C were significantly higher than those in group D at T_2(P0.05).The levels of urinary calcium in group B and C were lower than that in group A at T_2(P0.05).However,there were no apparent differences of the levels of serum calcium and urinary creatinine between groups at T_2(P0.05).Conclusion Calcifying nanoparticles could cause renal tubular epithelial cells injury,which leads to the formation of kidney stones.
【Fund】: 四川省科技厅资助项目(14JC0105);; 四川省卫生厅资助项目(100288)
【CateGory Index】: R692.4;R-332
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Chinese Journal Full-text Database 2 Hits
1 YU Cheng-fan,HUANG Xiao-bo,CHEN Liang,XU Qing-quan,HU Wei-guo,WANG Xiao-feng(Department of Urology,Peking University People's Hospital,Beijing 100034,China);Effect of nanobacteria on cell damage and crystal retention in renal tubular epithelial cells[J];Journal of Peking University(Health Sciences);2010-04
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1 Tang Jinyuan,Zhou Zhansong,Shen Xuecheng,Yuan Yufeng(Institution of Urology,Southwest Hospital,Third Military Medical University,Chongqing,400038,China);Rapid detection and clinical evaluation of nanobacteria infection in type Ⅲ prostatitis[J];Journal of Third Military Medical University;2011-06
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