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《Chongqing Medicine》 2016-03
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Estradiol induce neural proliferation and differetiation through regulating the expression of BDNF

Chen Qiong;Lin Liying;Tang Xiaohua;Guo Xuguang;Xia Yong;Department of Laboratory Medicine,the Third Affiliated Hospital,Guangzhou Medical University;  
Objective To address the influences of estradiol on the proliferation and neuronal differentiation in neural tissues,and then analyse whether BDNF involves in the pathway of this estradiol-induced cell division and specification.Methods The forebrain tissues of experimental animals were cultured in vitro,and treated respectively with estradiol,estrogen receptor inhibitor,BDNF,BDNF antibody,or received a combined treatment.And the immunohistochemistry was performed to reveal the situation of cell proliferation,neuronal differentiation in different experimental groups.Then real-time PCR was carried out to detect whether the expression of BDNF was regulated by estradiol.Results Both estradiol and BDNF increased the newborn cells and Hu positive neurons in cultured tissues,estrogen receptor inhibitor tamoxifen and BDNF antibody decreased the measurements conversely;the effects of estradiol were diminished by anti-BDNF,while BDNF′s effect didn′t changed by tamoxifen.Moreover,the expression of BDNF mRNA were up-regulated by estradiol and were inhibited by estrogen receptor inhibitor.Conclusion Estradiol might induce neural proliferation and differentiation in vitro by regulating the level of BDNF.
【Fund】: 广州市医药卫生科技项目(20131A011160);; 广州医学院第三附属医院博士启动项目(2012Y19)
【CateGory Index】: R392.2
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