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《Chongqing Medicine》 2016-03
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The preliminary research for the corticosterone to the femoral head microenvironment of rat 11 beta HSD1 expression and the influence of bone reconstruction

Zhao Jia;Wei Bo;Liu Jun;Liang Rongban;Huang Tao;Xie Fengwei;Huang Xianjin;Feng Dehui;Department of Emergency,Affiliated Hospital of Guangdong Medical College;Department of Orthopedic,Affiliated Hospital of Guangdong Medical College;Geriatric Department,Affiliated Hospital of Guangdong Medical College;  
Objective To study the endogenous glucocorticoid on rat femoral head microenvironment of 11 hydroxysteroid dehydrogenase expression,and to discuss the influence of combined with femoral head pathological changes of the corresponding mechanism.Methods Sixty SD rats were divided into control group,1-month group,3-months group,each 20 rats in group.1-month group and 3-months group inject cortisone acetate in the abdominal cavity intraperitoneal for 1-month or 3-months each.Immunohistochemical,immunofluorescence,Real-time qPCR,HE staining were employed in this study.Results From immunohistochemical,immunofluorescence,Real-time qPCR,the 11 hydroxysteroid dehydrogenase content of 1-month group and 3-months group were higher than that of the control group(P0.05).From HE staining we detected 1-month group in the bone marrow cavity increased in fat cells,3-months group subchondral trabecular bone density decreased,compared with the control group(P0.05).Conclusion Supplement of corticosterone could promote rat femoral head microenvironment 11 hydroxysteroid dehydrogenase expression and subchondral trabecular bone density decrease.
【Fund】: 广东医学院附属医院优硕基金项目资助(20501YS011005)
【CateGory Index】: R965
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