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《Chongqing Medicine》 2017-02
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Effect of WTX gene on colon cancer cell proliferation and apoptosis

Cao Mengyuan;Bai Yang;Xu Ying;Medical Journal of Jilin Province;Department of Ultrasound,First Hospital,Jilin University;Department of Nephrology,First Hospital,Jilin University;  
Objective To use WTX gene for transfecting into Lovo colon cancer cell line,and to analyze the impact of WTX gene on colon cancer cell proliferation and apoptosis.Methods Lovo colon cancer cell line was divided into the WTX gene transfection group and control group.The WTX gene transfection group was given WTX plasmid transfection,while the control group received empty vector transfection.The expression of WTX gene in the WTX gene transfection group and control group was observed.The proliferation,apoptosis and cell cycle change of Lovo colon cancer cells in two groups were also observed.Results The WTX protein expression in the WTX gene transfection group was significantly higher than that in the control group(P0.05);the OD values on 4,7dafter transfection in the WTX gene transfection group were significantly lower than those in the control group(P0.05);the G1 phase cells proportion in the WTX gene transfection group was significantly higher than that in the control group(P0.05),the G2 and S phase cells proportion in the WTX gene transfection group was significantly lower than that in the control group(P0.05).Conclusion WTX gene inhibits the proliferation of colon cancer cells,affects colon cancer cells cycle,has no effect on apoptosis of colon cancer cells.
【Fund】: 吉林省科技发展计划项目(2013052218JH)
【CateGory Index】: R735.35
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