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《Chongqing Medicine》 2017-02
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Expression up-regulation of lncRNA BANCR in hepatocellular carcinoma tissue and its prognostic value on patients′disease

Zhao Ji;Zhou Chao;Li Hongmin;Yang Hongji;Organ Transplantation Center,Sichuan Provincial People′s Hospital;Department of Gastroenterology,Sichuan Provincial People′s Hospital;Department of Oncology,Sichuan Provincial People′s Hospital;  
Objective To investigate the expression up-regulation of BRAF activated LncRNA(BANCR)in hepatocellular carcinoma(HCC)tissue and its prognostic value on patients′disease.Methods The quantitative real-time PCR(qRT-PCR)was used to detect BANCR expression in HCC tissues and cell lines.The association between BANCR expression level and clinicopathological features was also analyzed.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT),flow cytometry,and transwell invasion and migration assays were used to investigate the biological function of BANCR in the HCC cells.Results Compared with adjacent noncancerous tissues,BANCR expression was remarkably increased in HCC tissue sample(P 0.01),moreover BANCR expression in HCC cell lines was also significantly up-regulated(P0.05).The clinicopathologic features analysis revealed that the BANCR expression increase was closely correlated with higher tumor grade,tumor size,venous infiltration and higher TNM staging(P0.05).The total survival period in HCC patients with BANCR overexpression was significantly shorter than that in the patients with BANCR low expression(P0.01).The multi-variate Cox regression analysis indicated that the BANCR expression(RR=4.245,P=0.015),tumor diameter(RR=2.655,P=0.039),venous invasion(RR=3.278,P=0.022)and TNM staging(RR=6.379,P=0.006)were the independent prognostic factor of the total survival period in the HCC patients.Moreover,BANCR expression down-regulation in Hep3 Bcells could significantly inhibit the cellular invasion and transfer,meanwhile led to vimentin down-regulation and E-cadherin up-regulation.Conclusion This research results suggest that BANCR may contribute to HCC initiation and development process and would be used as a marker of the prognosis in HCC patients as well as a therapeutic target.
【CateGory Index】: R735.7
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