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《Chongqing Medicine》 2017-02
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Application of vitamin C combined with bortezomib in human T cell lymphoma cell line Jurkat cell apoptosis

Xing Hongyun;Bian Tierong;Deng Yu;Department of Hematology,Affiliated Hospital,South West Medical University;Material College,Xi′an University of Technology;Second Department of Clinic,Chongqing Medical University;  
Objective To study the influence of vitamin C combined with bortezomib on the apoptosis regulatory protein B lymphocytoma-2(Bcl-2)and Bcl-2associated X protein(Bax)expression regulation in human T-cell lymphoma cell line Jurkat cells.Methods 30nmol/L bortezomib group,100μg/mL vitamin C group and the combination group were adopted to act on Jurkat cells for 24,48,72 hrespectively.There was no drug added to the control group.The inverted microscope was used to observe the cell growth way.The expression of Bax and Bcl-2proteins was detected by immunohistochemistry.CCK-8and flow cytometry were used to detect the cellular apoptosis and cellular cycle.Results Vitamin C made the Jurkat cell growth way to change from the local accumulation growth in the control group to almost evenly disperse change.The expression decrease of Bcl-2from 24 hto 48hin the vitamin C group and combination group were faster than that in the bortezomib group,the Bax expression increase was also faster,moreover the expression increment of Bax from 24 hto 48hby vitamin C far surpassed that in the bortezomib group.The inhibition rates of Jurkat cell in three groups detected by CCK-8at 72 hwere 25.72%,75.23% and 56.81%respectively.The apoptosis rates at 72 hin three groups detected by flow cytometry were 81.73%,67.06% and 81.50% respectively.Early apoptosis rate in the vitamin C group was greater than the late apoptosis rate,but the bortezomib group and combination group all had low early apoptosis rate,but cell early apoptosis rate in the combination group was increased by about 15.26%after adding vitamin C.Conclusion Vitamin C inhibits T-cell lymphoma cell line Jurkat cell proliferation,especially promotes early apoptosis of Jurkat cells,moreover confirms that the apoptosis regulatory proteins Bax and Bcl-2participate in the vitamin C-induced Jurkat cell apoptosis mechanism.
【Fund】: 四川省卫生厅项目(110349)
【CateGory Index】: R73-36
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【Citations】
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1 JIANG Li-Cai;LUO Jian-Min;Department of Hematology,Xingtai People Hospital;Department of Hematology,The Secand People Hospital of Hebei Medical University;;Effect of Bortezomib on Proliferation,Apoptosis and SHP-2 Gene Expression of Lymphoma Cells[J];中国实验血液学杂志;2015-04
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Xing Hongyun;Bian Tierong;Deng Yu;Department of Hematology,Affiliated Hospital,South West Medical University;Material College,Xi′an University of Technology;Second Department of Clinic,Chongqing Medical University;;Application of vitamin C combined with bortezomib in human T cell lymphoma cell line Jurkat cell apoptosis[J];重庆医学;2017-02
2 LIANG Bo;YIN Jun-Jie;WANG Zhong-Liang;ZHAN Xin-Rong;Department of Hematology,Central Hospital in Xinxiang City;;Clinical Comparative Study of Two Kind Doses of Bortezomib Combinated with Bisphosphonates for Treating Patients with Multiple Myeloma Ostespathy[J];中国实验血液学杂志;2016-03
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