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《Chongqing Medicine》 2017-02
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Role of p38 MAPK signal pathway in TLR4 promoted angiogenesis of pancreatic cancer

Sun Yunliang;Ma Jianxia;Man Xiaohua;Wu Hongyu;Department of Gastroenterology,Ganyu District People′s Hospital;Department of Gastroenterology,Affiliated Huadong Hospital,Fudan University;Department of Gastroenterology,Affiliated Changhai Hospital,Second Military Medical University;  
Objective To investigate the role of Toll like receptor-4(TLR4)and p38mitogen-activated protein kinase(p38MAPK)signaling pathway in the angiogenesis of pancreatic neoplasms.Methods The in vitro cultured pancreatic cancer PANC-1cells were treated with lipopolysaccharide(LPS),TLR4-siRNA and p38 MAPK signal pathway inhibitor SB203580.TLR4,vascular endothelial growth factor(VEGF),p-p38 protein expression of PANC-1cells were detected by Western blot.The culture supernatants of PANC-1cells after various factors treatment were collected for observing their effects on the proliferation,migration and tube formation of human umbilical vein endothelial cells(HUVECs).Results The proliferation rate,number of migration and tube formation of HUVECs in the LPS group were(139.2±12.6)%,48.1±9.1and 47.8±9.6,respectively,which were significantly higher than those in the control group(P0.05).The proliferation rate,number of migration and tube formation of HUVECs in the TLR4-siRNA group were(60.2±8.7)%,31.3±4.5and 17.2±3.3,respectively,which were significantly lower than those in the control group(P0.01).The proliferation rate[(79.6±8.9)],number of migration(21.6±4.3)and tube formation(23.5±4.3)of HUVECs in the SB203580 group were significantly lower than those in control group(P0.05),moreover the proliferation rate,number of migration and tube formation of HUVECs in the TLR4-siRNA+LPS and SB203580+LPS groups were significantly lower than those in the LPS group(P0.01).Compared with the control group,VEGF and p-p38 protein expression in the LPS group were significantly increased,and TLR4,VEGF and p-p38 protein expression in the TLR4-siRNA and SB203580 groups were decreased;moreover VEGF and p-p38 protein expression in the TLR4-siRNA+LPS and SB203580+LPS groups were significantly decreased compared with the LPS group.Conclusion TLR4 may promote the angiogenesis in pancreatic cancer,its mechanism is related with activating the p38 MAPK signaling pathway and promoting VEGF expression.
【Fund】: 连云港市卫生局科研项目(1427);; 江苏省卫生厅科研项目(Y201420)
【CateGory Index】: R735.9
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 SUN Yun-liang,MAN Xiao-hua,WANG Li-hua,WU Hong-yu,LI Shu-de(Department of Gastroenterology,Ganyu Hospital,Ganyu Jiangsu 200433,China);Correlation of TLR4 expression with invasion and metastasis of pancreatic cancer[J];中国癌症杂志;2012-07
2 MAN Xiao-hua,SUN Yun-liang,GONG Yan-fang,WU Hong-yu,LI Zhao-shen.Department of Gastroenterology, Changhai Hospital,Second Military Medical University,Shanghai 200433,China;Relationship between TLR4 expression and angiogenesis in human pancreatic adenocarcinoma[J];中华胰腺病杂志;2012-03
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 Xu Zeng-xiang;Wang Jian-jun;Li Xiao-min;Lu Lin-ming;Department of Pathology,Wannan Medical College;Department of Histology and Embryology,Wannan Medical College;;Expression of TLR4 in Colon Cancers and Its Relationship with Tumor Proliferation and Metastasis[J];世界最新医学信息文摘;2017-13
2 Sun Yunliang;Ma Jianxia;Man Xiaohua;Wu Hongyu;Department of Gastroenterology,Ganyu District People′s Hospital;Department of Gastroenterology,Affiliated Huadong Hospital,Fudan University;Department of Gastroenterology,Affiliated Changhai Hospital,Second Military Medical University;;Role of p38 MAPK signal pathway in TLR4 promoted angiogenesis of pancreatic cancer[J];重庆医学;2017-02
3 Sun Yunliang;Yu Yang;Tong Yili;Wu Hongyu;Ma Jianxia;Department of Gastroenterology,The People's Hospital of Ganyu County;;Relationship between TLR4 and the sensitivity of pancreatic cancer PANC1 cells to gemcitabine[J];中华胰腺病杂志;2016-04
4 Wang Wei;Wu Yanhu;Zhu Jingfu;Liu Xiang;Zhang Shijiang;Department of Cardiothoracic Surgery,the First Affiliate Hospital of NJMU;;Relationship between TLR4 expression and angiogenesis in human AEJ[J];南京医科大学学报(自然科学版);2015-05
5 LIU Shasha;LIU Bingrong;Department of Gastroenterology,the Second Affiliated Hospital of Harbin Medical University;;The research of TLR4,TLR9 and colorectal cancer[J];胃肠病学和肝病学杂志;2015-03
6 Sun Yunliang1,Ma Jianxia2,Wu Hongyu3,Man Xiaohua3,Li Shude3(1.Department of Gastroenterology,People's Hospital of Ganyu,Ganyu 222100,Jiangsu,China;2.Department of Gastroenterology,Huadong Hospital Affiliated to Fudan University,Shanghai 200338,China;3.Department of Gastroenterology,Changhai Hospital Affiliated to Second Military Medical University,Shanghai 200433,China);Inhibitory effect of triptolide on human pancreatic cancer PANC-1 cells and its possible mechanism[J];中国肿瘤生物治疗杂志;2013-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Ilyar Sheyhidin, Julaiti Ainiwaer, Department of Thoracic Surgery of the First Affiliated Hospital, Medical University of Xinjiang, 830054 Urumqi, Xinjiang, China Gulnaz Nabi, Department of Rehabilitative of the First Affiliated Hospital, Medical University of Xinjiang, 830054 Urumqi, Xinjiang, China Ayshamgul Hasim, Rui-Ping Zhang, Hong Ma, Hua Wang, Department of Pathology, College of Basic Medicine, Medical University of Xinjiang, 830011 Urumqi, Xinjiang, China;Overexpression of TLR3, TLR4, TLR7 and TLR9 in esophageal squamous cell carcinoma[J];World Journal of Gastroenterology;2011-32
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