Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chongqing Medicine》 2017-02
Add to Favorite Get Latest Update

Effect of long-chain non-coding RNA UBC1 on biological function of bladder cancer cells

Zhu Chenxi;Li Wenzhou;Guo Yonglian;Yu Jiajun;Shu Bo;Chen Lin;Li Guohao;Wei Dan;Department of Urology,Wuhan Municipal Central Hospital;  
Objective To investigate the effect of long-chain non-coding RNA-UBC1(lncRNA-UBCI)on proliferation,apoptosis,invasion and metastasis of bladder cancer cells and its possible mechanism.Methods The siRNA was used to silence lncRNAUBC1 in bladder cancer cell line T24 cells.The negative control group was set.CCK-8was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,Transwell assay was used to detect the invasion ability and the expression of EZH2 and MMP-2was detected by Western blot.Results The lncRNA-UBC1 expression in T24 cell lines was significantly higher than that in human embryonic bladder tissue derived cells.Compared with the negative control group and blank control group,T24 bladder cancer cell proliferation rate in the UBC1 silence group was slower than that of the control groups.T24 bladder cancer cell apoptosis cell percentage in the UBC1 silence group was higher than that in the negative control group[(18.72±7.79)% vs.(13.09±5.66)%,P0.05].The T24 bladder cancer cell invasion ability in the UBC1 silence group was inhibited.Expression of MMP-2and EZH2 of T24cell in UBC1 silence group was decreased.Conclusion Silencing lncRNA-UBC1 can inhibit the proliferation,apoptosis and invasion ability of T24 cells and its mechanism may be through MMP-2and EZH2 pathway.
【CateGory Index】: R737.14
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Zheng Wenwen;Zhang Shufang;Central Laboratory,Haikou People's Hospital;;The research progress of long non-coding RNA in bladder cancer[J];现代肿瘤医学;2015-24
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 CHI Hong;CHEN Junxia;Department of Cell Biology and Genetics, Chongqing Medical University;;The role of lncRNA RP11-79H23.3 in bladder cancer cells and its mechanism[J];中国癌症杂志;2018-01
2 LIU Qing;LIANG Meihua;ZHANG Xing;LI Zhengzhao;CAO Jun;Department of Oncology,the First People′s Hospital of Qujing,Yunnan Province;Laboratory of Tumor Immune and Micro Environmental Regulation,Guilin Medical University;Department of Emergency,West Brance,the First Affiliated Hospital of Guangxi Medical University;;Effects of formononetin on proliferation and apoptosis of human bladder cancer cells T739 and its mechanism[J];中国医药导报;2017-25
3 Zhu Chenxi;Li Wenzhou;Guo Yonglian;Yu Jiajun;Shu Bo;Chen Lin;Li Guohao;Wei Dan;Department of Urology,Wuhan Municipal Central Hospital;;Effect of long-chain non-coding RNA UBC1 on biological function of bladder cancer cells[J];重庆医学;2017-02
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Feng Yang;Fan Yi;Huiqing Cao;Zicai Liang;Quan Du;Institute of Molecular Medicine, Peking University;;The emerging landscape of long non-coding RNAs[J];遗传;2014-05
2 YU Meng1,ZHU Yu-yan2,YANG Chun-ming2,WANG Hai-bo2,LI Yuan-yuan3,NAKAGAWARA Akira3,ZHENG Zhi-hong1(1.Department of Reproductive Biology and Transgenic Animals,China Medical University,Shenyang 110001,China;2.Department of Urology,The First Hospital,China Medical University,Shenyang 110001,China;3.Laboratory of Cancer Genomics,Chiba Cancer Center Research Institute,Chiba 260-8717,Japan);Screening and Identification of Candidate Genes Associated with Progression of Neuroblastoma[J];中国医科大学学报;2012-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved