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《Chongqing Medicine》 2017-02
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Effects of two different cell culture medium on proliferative and functional activities of cytokine-induced killer cells

Song Wenling;Han Rong;Zhang Man;Zhang Quan;Yao Weiqi;Wu Dongcheng;Third Department of Oncology,Huangpi District People′s Hospital,Third Affiliated Hospital,Jianghan University;Department of Research and Development,Wuhan Hamilton Biotechnology Co.,Ltd;Tumopr Center,First Clinical College of Wuhan University,Wuhan University;School of Basic Medicine Sciences,Wuhan University;  
Objective To observe the proliferation ability of cytokine-induced killer(CIK)cells and to detect surface molecule phenotype of CIK cells and in vitro killing effect to leukemia K562 cells.Methods The cord blood lymphocytes and peripheral blood mononuclear cells(PBMCs)from patients were cultured by the H3 medium and added with human recombinant interferon(IFN)-γand CD3 monoclonal antibodies(mAb)for inducing CIK cells in vitro.The H3 medium and T551 medium were respectively added on 7dof culture.The induction and culture were continued until 14 d.The CIK cells proliferation ability was observed by cell count and the expressions of CD3 and CD56were detected by flow cytometry.The killing effect of CIK cells on leukemia cells was tested by CCK8 assays.Results The dynamic counting and phenotype analysis results revealed that the CIK cells amplification times(including cord blood CIK total cells and autologous CIK total cells)cultured by H3 and H3+T551reached 76.9times and62.3times on 14drespectively;the CD3+CD56+double positive cells contents of cord blood CIK cells under the two kinds of culture condition were(16.7±2.7)% and(10.8±2.6)% respectively,while which of autologous CIK cells were(11.2±6.6)% and(10.7±6.4)% respectively;the in vitro killing-tumor test revealed that when the effector to-target ratio was 5:1,the cell cytotoxic activity of cord blood CIK cells cultured by H3reached(33.50±9.99)%,which was significantly higher than(20.3±6.76)% of cord CIK cells cultured by H3+T551(P=0.011),while which of autologous CIK cells cultured by H3+T551 were(59.67±27.59)% and(42.13±19.47)% respectively,but the difference was not significant(P=0.080).Conclusion Cord blood and autologous CIK cells incubated by H3 have stronger in vitro anti-leukemia cells activity and can be used in the adoptive immunotherapy of leukemia in clinic.
【CateGory Index】: R730.51
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【Citations】
Chinese Journal Full-text Database 4 Hits
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