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《Chongqing Medicine》 2017-05
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Comparative characterization of osteo/odontogenic differentiation capability of human dental pulp stem cells and stem cells from apical papilla in vitro

Tan Xiaobing;Guo Yu;Liu Jia;Xu Jingshu;Dai Qingyuan;Department of Cariology and Endodontics,the First people′s Hospital of Yunnan Province;Department of Cardiology,the First Affiliated Hospital of Kunming Medical University;  
Objective To compare the growth characteristics,proliferation and osteo/odontogenic differentiation capability of stem cells from human dental pulp(dental pulp stem cells,DPSCs)and apical papilla(stem cells from apical papilla,SCAP)in vitro.Methods Human dental pulp and apical papilla tissues were separated from impacted third molars of young healthy donors at the age of root development and digested by collagenase type Ⅰ and dispase type Ⅱ to derive primitive DPSCs and SCAP.Cells were then induced for osteo/odontongenic differentiation by medium containingβ-glycerophosphate,dexamethasone and KH2PO4.Flow cytometry was utilized to test the expression of specific markers of stem cells,including CD24,CD34,CD45,CD90,CD105,CD146,STRO-1and OCT-4.AR-S was used to display the mineralization structure and RT-PCR was applied to analyze the expression of bone sialoprotein(BSP),osteocalcin(OCN)and dentine sialophosphoprotein(DSPP).Results Both DPSCs and SCAP were positive for CD90,CD105,CD146,STRO-1and OCT-4,in percentages varying according to cell type,without expression of CD34 or CD45.Only SCAP expressed CD24 positively.Both cells formed organized mineralization structure after 2 weeks of induction in time-dependent manner,with more mineralization by SCAP and expressed differentiation markers,including BSP,OCN and DSPP.Conclusion Human DPSCs and SCAP possess the characteristics of MSCs and could be differentiated into odontonblast-like cells in vitro.Both cells are approachable stem cell sources for dental tissue engineering.
【Fund】: 国家自然科学基金资助项目(81360161);; 云南省教育厅基金资助项目(2015Y153)
【CateGory Index】: R781
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